Plating Media: R medium + 2 uM metatopolin + 4, 1cm3 cubes Russet potatoes. 60 mins. bleaching with 0.525% Chlorox bleach
Replating Media: R medium + 1, 1cm3 cube Russet potato
Number of Days between Plating and Replating: 65 days
Number of Days between Replating and Vernalization: 253 days
Number of Days between start and end of Vernalization: 120 days (4 months)
Date to Initiate Plating: Jan. 15
Date to Replate: March 23
Date to start to Vernalize: Dec. 1
Results:
2010
Seedlings original plated on Nov. 10,2009, replated on Jan. 14, 2010 and began vernalization on Sept. 27, 2010 have grown well with shoots about 1/2 to 3/4 inches tall with 2-4 roots 3 inches long. As indicated above need to begin plating much earlier to avoid having the seedlings in vernalization for 6 months. This promotes fungused seedlings. The plating and replating media works very well. Stick with it. The seeds germinate very quickly.